Part:BBa_K1378009
INPNC-Linker (rTEV)-MVN & GFP
Introduction
This plasmid is an advanced testing plasmid for our E. coli - M. aeruginosa binding assay. It differs with the part BBa_K1378007 in that we added a special linker between INPNC and MVN. We inserted two transcription units into one plasmid and they are expressed under the promoters J23105 and J23117 respectively. After this plasmid is transformed into E. coli, the mannan binding protein MVN (microvirin) will be displayed on the cell surface by INPNC (Ice Nucleation Protein N and C terminal) and the E. coli cell will express GFP as well. There is a linker between INPNC and MVN that can be specifically digested by rTEV protease so we can add rTEV protease to control the binding activity of our E. coli cells. The cell culture with rTEV protease added can serve as the control group for the binding assay.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1528
Illegal NheI site found at 1551 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 466
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2229
Illegal SapI site found at 1071
Characterization
We did not carry out the binding assay for this part. Detailed characterization of INPNC-MVN can be found in the part BBa_K1378007.
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